Application

blood production, hematopoietic growth and differentiation, pathways of pluripotent cell differentiation into bone, cartilage and fat, connective tissue production,supportive structure, cell production, cell proliferation, cell based therapy, tissue restoration and repair, differentiation, effects of culture conditinos, HLA expression, cytokine production, immunomodulation, clinical trials

Cell Line Origin

Bone Marrow

Components

Basal Medium containing 10% FBS & 10% DMSO

General description

Bone marrow is the major blood creating organ, but in addition to supporting hematopoietic growth and differentiation, marrow stromal cells can be induced to produce cells of other connective tissues, such as bone, cartilage, and fat, as well as cells from neuroectodermal (neurons) and endodermal (hepatocytes) lineages. The potential of HMSCs to maintain multipotency and proliferate extensively in vitro provides new avenues for cell-based therapy in the restoration of damaged or diseased tissue. HMSC have been utilized to investigate the effects of TGF-β1 on differentiation toward different lineages, and show that it inhibits adipogenic differentiation and induces either chondrogenic, osteogenic or smooth muscle cell differentiation, depending the specific culture conditions (Zhao, 2011); to develop methods to assess differentiation potential of stem cells without the differentiation induction (Hirota, 2011); to compare HLA expression profiles of bone marrow-derived and adipose-derived stem cells (Purandare, 2013); and to design heart valve-shaped tri-layered collagen-based constructs for stem cell seeding and differentiation to use in heart valve tissue engineering (Tedder, 2010, 2011).

Preparation Note

2nd passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO Can be cultured at least 10 doublings

Subculture Routine

Please refer to the HMSC Culture Protocol.